LOCUS       40924_44804       10031 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pUB-GFP DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, 
            Hayashi M.
  TITLE     Polyubiquitin Promoter-Based Binary Vectors for Overexpression and 
            Gene Silencing in Lotus japonicus
  JOURNAL   Mol. Plant Microbe Interact. 21 (4), 375-382 (2008)
  PUBMED    18321183
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   Maekawa T, Hayashi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2007) Contact:Makoto Hayashi National Institute of
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan
REFERENCE   3  (bases 1 to 10031)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10031)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant 
            Microbe Interact."; date: "2008"; volume: "21"; issue: "4"; pages: 
            "375-382"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2007) Contact:Makoto Hayashi National Institute of 
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(103..277)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(300..1016)
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        complement(1067..1744)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    1935..1956
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1971..2001
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2009..2025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2033..2049
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2071..2323)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2370..2386)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(2387..2973)
                     /gene="Ljubq1"
                     /label=Ljubq1
     intron          complement(2387..2926)
                     /gene="Ljubq1"
                     /note="5' UTR intron of Ljubq1 gene"
     exon            complement(2927..2973)
                     /gene="Ljubq1"
                     /number=1
     regulatory      complement(2974..3508)
                     /label=Ljubq1 promoter
                     /note="Ljubq1 promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(3535..3551)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3754..3778
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5078..5704
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6141..7205
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7274..7468
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7812..7952
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8138..8726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8816..9607)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gagccgggcg gccgctttac
      301 ttgtacagct cgtccatgcc gtgagtgatc ccggcggcgg tcacgaactc cagcaggacc
      361 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
      421 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      481 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      541 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
      601 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      661 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      721 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      781 atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tgaaggtggt cacgagggtg
      841 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
      901 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      961 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggga
     1021 tcgaattcct cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct
     1081 ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt gggattgtgc
     1141 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     1201 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     1261 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     1321 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     1381 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     1441 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca
     1501 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     1561 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     1621 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     1681 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     1741 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     1801 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg
     1861 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     1921 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     1981 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2041 agctatgacc atgattacga attcgagctc gatctagtaa catagatgac accgcgcgcg
     2101 ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt
     2161 gcgggactct aatcataaaa acccatctca taaataacgt catgcattac atgttaatta
     2221 ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag
     2281 gattcaatct taagaaactt tattgccaaa tgtttgaacg atcgatggcg cgcctaggta
     2341 ccggatccac tagtaggcct tctagggggg atccactagt tctagactgt aatcacatca
     2401 acaacagata aatcagaatc caacacacga aattgaaaca agtgaaaacc ctaattaacg
     2461 cttggatcaa acagattaag agtggatcta acatcaacag ataaatcaga atccataaca
     2521 agaaattgaa acaagtgaaa accctaattg aagcttggat caaacagatt aagagtggat
     2581 ctaacaataa tttccaattt gaataaatta tttgacataa ttccataacg gatctgaaga
     2641 attcaattgg aaaccctaat taatctgcaa ttgctaaacc gaaatcgatt cctaacaaaa
     2701 ctgaaccaca attatcatag atctacatcg caagccatcg aaaacattga taaaacaaga
     2761 tctgagattc aagctgtaaa attcaatttc aaaccgatct caattttgga aaatcacaaa
     2821 aaattcctag atctcatgct tcgaaacaag aaaactacct atgattgatc ggaaacaaaa
     2881 ctcaacgaaa ggattacaga gaaagaagag agaaattgga tcataccttg aaagtagaga
     2941 gaagcgatat gaacaagaat tgttagggtt gggaactgaa ttgtgaagaa gctaagggga
     3001 ttggaggtaa tttatagcaa aagcggtgaa attattaacc agtcaaattg tgacacgtgt
     3061 ccttatgacg cgtcactagt tgcgtgtggt ttgggatagg atacgttgtg tggttgagtt
     3121 cttcttggaa acggtagttg caaattgcaa gttgttggcg gtttgttaat ttcacggagt
     3181 taaacgcatc tcgccgcctt ttgagtttgg attgataaat cgttacaacg gcgttacttt
     3241 gctgggcccc gttttttttg aattgggcca ccgcaccagt gcaagttggg ggttccttgg
     3301 ttttccagaa tgggctccac tctctatgat tgattggagg aggccggctg ctcagaaatt
     3361 ttataataag ttgttatata cttctcccct taaaataagt gatttttgtc tatccatagt
     3421 tgcaataagt tgtactaaca acttatctaa attactttat caataataca agtaattcaa
     3481 ttaattattt cctcaaaatc ctctctccat cgaattcctg cagcccagct tggcactggc
     3541 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     3601 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     3661 ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt
     3721 cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct
     3781 aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc
     3841 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     3901 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     3961 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     4021 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     4081 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     4141 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     4201 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     4261 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     4321 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     4381 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     4441 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     4501 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     4561 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     4621 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     4681 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     4741 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     4801 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     4861 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     4921 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     4981 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     5041 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     5101 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     5161 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     5221 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     5281 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     5341 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     5401 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     5461 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     5521 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     5581 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     5641 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     5701 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     5761 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
     5821 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
     5881 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
     5941 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
     6001 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
     6061 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa
     6121 gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     6181 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
     6241 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     6301 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     6361 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     6421 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     6481 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
     6541 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     6601 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     6661 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     6721 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     6781 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     6841 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     6901 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     6961 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     7021 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     7081 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     7141 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     7201 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     7261 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     7321 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     7381 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     7441 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
     7501 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     7561 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     7621 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
     7681 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     7741 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     7801 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     7861 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     7921 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     7981 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8041 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8101 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8161 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8221 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8281 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8341 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8401 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8461 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8521 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     8581 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8641 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8701 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8761 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca
     8821 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
     8881 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga
     8941 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
     9001 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
     9061 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
     9121 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
     9181 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
     9241 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
     9301 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
     9361 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
     9421 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
     9481 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
     9541 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
     9601 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
     9661 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
     9721 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
     9781 tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
     9841 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
     9901 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
     9961 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
    10021 tggctggctg g
//
